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Old 12-12-2013, 03:28 AM
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G.Alexander G.Alexander ثeu
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Default Diluting ZeoStart3 for Automated Dosers by NanoTopia

By diluting ZeoStart3 solution you can more accurately dose smaller amounts of Zeostart3 in an automated way via peristaltic doser. This prevents possible missed doses and improves consistency and delivery of the carbon source to sustain balanced amounts in the system.

I have been using this method for a year with good results.

Consider the following mixing ratios first.

1:19, ZeoStart3/RODI water. 1 part ZeoStart3 (say 100mL) to 19 parts RODI water (say 1900mL) will yield you 2000mL of solution. Each 1mL of solution will contain 0.05mL of ZeoStart3.

e.g.
If you wanted to dose a total of 0.5mL per day of ZeoStart3 divided into 2 doses (0.25mL x 2), you would set your doser to deliver 5mL twice a day (5mL x 2 = 10mL of solution) = 0.5mL of ZeoStart3.

1:9, ZeoStart3/RODI water. 1 part ZeoStart3 (say 100mL) to 9 parts RODI water (say 900mL) will yield you 1000mL of solution. Each 1mL of solution will contain 0.1mL of ZeoStart3.

e.g.
If you wanted to dose a total of 0.5mL per day of ZeoStart3 divided into 2 doses (0.25mL x 2), you would would not be able to do it because most dosers deliver only 1mL minimum and 1 mL of solution contains 0.1mL of ZeoStart3. So this Ratio only works if you are dosing even numbers daily (like 0.2/0.4/0.6/0.8 mL and so on), get it? The 1:9 ratio is better when dosing larger amounts of ZeoStart3 with even numbers. You could dose more than 2 times daily to try and equal the total amount dosed per day but that just makes thing more difficult.

As long as you stick to the ratio, any amount of solution can be created. I recommend only making the amount that will fit in your dosing container at a time. Prolonged storage of the solution only increases the risk of contamination over time.

To dilute ZeoStart3 properly, follow this:

Depending on the accuracy of your doser (most are not that accurate) I recommend 1:19 ratio for dilution. A 1:9 ratio can also be used but I would only consider this ratio if your dose amounts will be over 5mL per dose.

It is also a good idea to calibrate your doser before beginning with RODI water. This will avoid wasting ZeoStart3 solution and risking overdosing the tank. Dosers should be calibrated and tested frequently for accuracy of dose.

The goal is not to introduce bacteria to your solution, this will cause contamination as the bacteria feed off the solution and multiply over time. This will generally cause the solution to become cloudy. To avoid this I recommend following the below steps as best as possible.

You will need a container to hold the solution that seals and can withstand temperature sterilization, usually glass works well.

Boil some RODI water and wash the inside of the container, lid, and all tubing (used for drawing the solution to the doser and tank.) Discard this water, do not dry with a cloth, do not touch any of the surfaces that will have contact with the solution. Place the items on a clean or sterilized surface to cool naturally.

Boil the required amount of RODI water to be used for your solution. Once at the boiling point remove from heat and allow to cool naturally. This may take some time so you can speed up the process by placing the boiling container in a ice bath (be sure not to contaminate your boiled water in this process). You should also cover the water to avoid what is called "bacterial rain", this is bacteria in the air that will fall to the surface of the water and contaminate it. It is best if you can complete the cooling within 30 minutes time to lower the chance of contamination.

Pour the cooled and measured amount of RODI water into your dosing container.

Add the measured amount of ZeoStart3 to the RODI water.

Place the lid and tube on the dosing container and swirl the solution for 60 seconds to mix it. You are now ready to connect the solution bottle to the dosing pump. Adjust the dosing pump to deliver the desired amount of ZeoStart3 solution twice a day. You can also dose 4 times a day but consider with a smaller dose delivery amount, the accuracy of the dose will also decrease. Most dosers will dose a minimum of 1mL, consider this when planning your dose amounts and frequency.

The solution will stay mixed without future or continual mixing. You could swirl it once a week if you are in doubt.

I recommend you check the actual amount of solution being delivered as the hose is normally positioned. This should be done on a regular basis just to make sure your doser is working properly. This can be done effectively by catching the solution as it is being delivered in a graduated cylinder. If you don't have a graduated cylinder, you could also use a syringe, remove the plunger and plug the hole where the needle would attach.
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